Cosmetic composition for increasing hyaluronic acid synthesis in skin and its use for repairing wrinkles

ABSTRACT

The present invention relates to a skin composition which has an excellent effect on smoothing or repairing wrinkles. The composition comprises Palmitoyl Tripeptide-38 (Palmitoyl-Lysyl-Dioxymethionyl-Lysine) and  Piper nigrum  (Pepper) seed Extract which combination has been found to result in a synergistic effect on hyaluronan synthase 1 (HAS1) mRNA expression in human fibroblasts. The hyaluronan synthase is responsible for the synthesis of hyaluronan (hyaluronic acid) in skin. Hyaluronic acid is a constituent of the extracellular matrix of the skin and responsible for space filling, tissue repair and retention of moisture.

This Application claims priority to European patent application no.12192675.2, filed Nov. 14, 2012, entitled “Cosmetic composition forincreasing hyaluronic acid synthesis in skin and its use for repairingwrinkles”, the entirety of which is incorporated herein by reference.

The present invention relates to a skin composition which has anexcellent effect on smoothing or repairing wrinkles. The compositioncomprises Palmitoyl Tripeptide-38(Palmitoyl-Lysyl-Dioxymethionyl-Lysine) and Piper nigrum (Pepper) seedExtract which combination has been found to result in a synergisticeffect on hyaluronan synthase 1 (HAS1) mRNA expression in humanfibroblasts. The hyaluronan synthase is responsible for the synthesis ofhyaluronan (hyaluronic acid) in skin. Hyaluronic acid is a constituentof the extracellular matrix of the skin and responsible for spacefilling, tissue repair and retention of moisture.

It was the problem of the present invention to provide an anti wrinklecomposition with superior wrinkles repairing effect.

It was found by the inventors of the present application that thisproblem can be solved with a cosmetic composition which comprises asactive substances

-   -   0,000025 to 0,001 wt% Palmitoyl-Lysyl-Dioxymethionyl-Lysine,    -   0,00105 to 0,063 wt% Piper nigrum (pepper) seed extract,    -   water and cosmetically acceptable auxiliaries, wherein the given        percentages relate to the total weight of the composition.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a chart showing the percentage of HAS-1 mRNA expression innormal human fibroblasts for various compositions; and

FIG. 2 shows the immunostaining results of this in vitro test on normalhuman skin.

It is known in the art that Palmitoyl-Lysyl-Dioxymethionyl-Lysine whichINCI name is Palmitoyl Tripeptide-38 stimulates the synthesis of skinmatrix molecules such as dermis collagen and epidermal hyaluronic acidand helps fill wrinkles. A commercially available product whichcomprises this tripeptide is Matrixyl®Synthe{grave over ( )}6™ (Sederma,France). The INCI name of Matrixyl®Synthe{grave over ( )}6™ is Glycerin(and) Water (and) Hydroxypropyl Cyclodextrin (and) PalmitoylTripeptide-38. The product comprises 0,025 wt % Palmitoyl Tripeptide-38,78,975 wt % glycerin, 1,0 wt % hydroxypropyl cyclodextrin and theremainder to 100 wt % water.

In a further embodiment of the invention the cosmetic compositioncomprises 0,000125 to 0,00075 wt % Palmitoyl Tripeptide-38 related tothe total weight of the composition, preferably 0,00025 to 0,00075 wt %.

Piper nigrum (Pepper) seed Extract is described in the art to beobtained from black pepper berries by powdering them and subjecting theaqueous solution of the powder to enzymatic hydrolysis. It is rich in inpolysaccharides, glucans and rhamnogalacturonans. A commerciallyavailable product which comprises this piper nigrum (Pepper) seedExtract is Retilactyl D® (Silab, France). This product comprises 2,1 wt% piper nigrum seed extract, stabilizer, preservative and 97,2 wt %water (INCI: Water (and) Piper nigrum (Pepper) seed extract). RetilactylD® was designed to help to mitigate the affects of photoageing onreticular fibroblasts.

In a further embodiment of the invention the cosmetic compositioncomprises 0,0021 to 0,0315 wt % Piper nigrum (Pepper) seed extractrelated to the total weight of the composition, preferably 0,0042 to0,021 wt %.

Surprisingly, it has now been found that the combination of PalmitoylTripeptide-38 and Piper nigrum (Pepper) seed extract has a synergisticeffect on synthesis of HAS1 mRNA in normal human fibroblasts. This waythe level of dermis hyaluronic acid in the skin is effectivelyincreased. Using e.g. 2 wt % Matrixyl®Synthe{grave over ( )}6™ (whichcorresponds to 0,0005 wt % Palmitoyl Tripeptide-38) and 0,5 wt %Retilactyl D® (which corresponds to 0,0105 wt % Piper nigrum seedextract) the HAS1 mRNA expression increased to 377%, whereas the HAS1mRNA expression of Matrixyl®Synthe{grave over ( )}6™ alone was 127% andthat of Retilactyl D® alone was 60% (compare example 4 and FIG. 1).

It has revealed that the anti wrinkle composition of the presentinvention can be further improved by adding Secale cereale (rye) seedextract and/or Kigelia africana fruit extract. By adding both activesthe HAS1 mRNA expression could be further improved to 401% (compare FIG.1).

Secale cereale (rye) seed extract is described in the art to be obtainedby subjecting the aqueous solution of rye flakes to enzymatichydrolysis. A commercially available product which comprises this ryeseed extract is Coheliss®Bio (Silab, France). The product comprises 6 wt% rye seed extract, 93,7 wt % water and 0,3 wt % preservative (INCI:Water & Secale cereale (rye) seed extract). The cosmetic composition ofthe invention comprises 0,003 to 0,18 wt % Secale cereale (rye) seedextract, related to the total weight of the composition, preferably0,006 to 0,09 wt %, more preferred 0,006 to 0,06 wt %.

Kigelia africana fruit extract is obtained from the fruits of theKigelia africana tree by hydroglycolic extraction. A commerciallyavailable product which comprises this extract is Kigeline (Greentech,France). The product comprises about 1,08 wt % Kigelia africana fruitextract, 39,0 to 39,9 wt % butylene glycol and the remainder to 100 wt%water (INCI: Water, Butylene Glycol, Kigelia africana fruit extract).The cosmetic composition of the invention comprises 0,000054 to 0,0324wt % Kigelia africana fruit extract related to the total weight of thecomposition, preferably 0,00108 to 0,0162 wt %, more preferred 0,00108to 0,0108 wt %.

The composition of the invention comprises auxiliary substances whichare selected from the group comprising one or more of emulsifiers,emollients, humectants, moisturizers, antioxidants, gelling agents,chelating or complexing agents, viscosity modulating agents, opacifiers,preservatives, colorants, fragrances, skin care agents, tanning agents,UV filters, buffers, solvents, and mixtures thereof.

The cosmetic composition of the invention is formulated as O/W or W/Oemulsion.

The emulsifiers which can be used for this purpose are well known by theskilled person. According to the invention examples of emulsifiers whichcan be used are: Polyacrylamide (and) C13-C14 Isoparaffin (and)Laureth-7 (Trade name: Sepigel 305), PEG-40 Stearate (Myrj S40),Glyceryl Stearate (Trade name: Cutina GMS V), PPG-1-PEG-9 Lauryl GlycolEther (Trade name: Eumulgin L), PEG-60 Hydrogenated Castor Oil (Tradename: Cremophor CO 60), Cetyl Alcohol and Glyceryl Stearate and PEG-75Stearate and Ceteth-20 and Steareth-20 (Trade name: Emulium Delta),Cetearyl Alcohol (Trade name: Nafol 1618), Hydroxyethyl Acrylate/SodiumAcryloyldimethyl Taurate Copolymer and water and Squalane andPolysorbate 60 and Sorbitan Isostearate (Trade name: Simulgel NS).

The composition according to the present invention comprises 0,01 to 3,0wt % antioxidants, preferably 0,05 to 1,0 wt %, more preferred 0,08 to0,3 wt %, wherein the given percentages relate to the total weight ofthe composition. Suitable antioxidants are for instance vitamin C andits derivates, e.g. ascorbyl acetate, ascorbyl phosphate, ascorbylpalmitate, vitamin E, derivates of vitamin E, a plant extract mixturecomprising Angelica Archangelica Root Extract, Camellia Sinensis LeafExtract, Pongamia Pinnata Seed Extract and Coffea Arabica Seed Extract(RPF complex), and mixtures thereof. For instance Oxynex K (Merck) whichis a mixture of tocopherol, ascorbyl palmitate, citric acid, ascorbicacid and PEG can be included into the present composition.

The RPF complex which may be used in the cosmetic composition of theinvention includes preferably extracts from green coffee beans, fromleaves of green tea (Camellia sinensis), from seeds of Pongamia pinnata,from roots of Angelica archangelica and from peel of Citrus aurantium(Bitter orange). The extracts are prepared by extraction with amonovalent or multivalent alcohol or a mixture of such alcohol(s) withwater at room temperature (about 15-30° C.). The extracts are used inliquid or dried form. An especially preferred plant extract mixture is amixture of liposomic encapsulated plant extracts of 1-4% CamelliaSinensis Leaf Extract, 1-4% Green Coffee Seed Extract, 1-4% PongamiaPinnata Seed Extract, 1-4 Angelica Archangelica Root Extract, 1-4%Citrus Aurantium Peel Extract, 2-10% phospholipids in anaqueous-alcoholic suspension wherein the alcohol content is in the rangeof 4-12%, wherein all concentrations are related to the weight of theplant extract mixture.

According to the invention emollients can be added to soften andsmoothen the skin. Suitable emollients are for instance Caprylic/CapricTriglyceride, Ethylhexyl Palmitate, silicones, such as dimethicone,Polysilicone-11, siloxanes, such as Cyclopenta-siloxane/Dimethiconol,Cyclohexasiloxane; Butyrospermum Parkii (Shea) Butter. Further suitableemollients are for example Cetearyl Isononanoate, Octyldodecanol,Isopropyl Palmitate, Isopropyl Myristate, Mineral Oils, Isohexadacane,Diisopropyl Sebacate, C12-15 Alkyl Benzoate, Propylheptyl Caprylate,Pentaerythrityl tetraisostearate, Cetearyl Isononanoate, IsononylIsononanoate, Ethyl Hexyl Hydroxystearate, Phenoxyethyl Caprylate,Isoamyl Cocoate.

In an advantageous embodiment of the invention the composition containsmoisturizing substances in a range of 0.1 to 10,0 wt %. Preferredmoisturizers which may be used are glycerine, propylene glycol andbutylene glycol. Additionally, hyaluronic acid microspheres (Trade name:Filling Spheres™ hyaluronic, BASF) may be added from 0,01 to 2 wt %,related to the total weight of the composition, preferably 0,01 to 0,2wt %, wherein the microspheres contain 0.2 wt % sodium hyaluronate.

The composition of the present invention has an excellent wrinklecorrecting effect, mainly based on increased dermis hyaluronic acidsynthesis in skin. Therefore, it is another object of the presentapplication to provide a composition for use in increasing hyaluronicacid synthesis in skin and to provide a composition for use in repairingor smoothing wrinkles, preferably facial wrinkles. Yet another object ofthe present invention is a method for treating wrinkles on skin,preferably facial wrinkles, by applying the composition of the presentinvention to skin areas with wrinkles to be treated.

The compositions of the present invention are prepared in a manner wellknown for O/W or W/O emulsions in cosmetic industries. Details of thepreparation are given in the examples.

The following examples are offered to illustrate the cosmeticcompositions of the present invention and their preparation. They arenot intended to be limiting in any respect.

EXAMPLE 1

DAY CREAM A B B INCI Name/description of ingredients % % % Phase 1 WATERq.s. q.s. q.s. COLORANT ACID RED 27 0.00015 0.00025 0.0005STYRENE/ACRYLATES COPOLYMER 0.8 1.40 1.80 (hydro) GLYCERIN 2.0 4.0 6.0POLYACRYLAMIDE, WATER, C13-14 4.9 4.7 4.5 ISOPARAFFIN, LAURETH 7 Phase 2HYDROGENATED POLYISOBUTENE 4.0 2.7 1.5 CYCLOPENTASILOXANE & 2.0 2.8 3.5DIMETHICONOL Phase 3 SECALE CEREALE (RYE) SEED 0.1 2.0 0.6 EXTRACT(hydro) (Coheliss ®Bio) KIGELIA AFRICANA FRUIT EXTRACT 0.15 2.5 0.6(hydroglycolic) (Kigeline) MICROSPHERES SODIUM 0.05 0.12 0.08HYALURONATE (Hyaluronic filling spheres) ENCAPSULATED INGREDIENTS RPF0.35 0.25 0.15 COMPLEX HYDROLYZED CITRUS AURANTIUM 1.5 1.0 0.7 DULCISFRUIT EXTRACT (hydro) MAURITIA FLEXUOSA FRUIT OIL 0.5 0.4 0.3 BIFIDAFERMENT LYSATE 0.1 2.0 0.6 GLYCERIN & WATER & 0.3 3.5 2.0 HYDROXYPROPYLCYCLODEXTRIN & PALMITOYL TRIPEPTIDE-38 (Matrixyl ®Synthe{grave over( )}6 ™) PIPER NIGRUM (PEPPER) SEED 0.25 2.0 0.45 EXTRACT (hydro)(Retilactyl D ®) Phase 4 NYLON-12 1.0 1.9 1.7 PTFE 1.5 1.2 1.5 Phase 5FRAGRANCE 0.1 0.25 0.4 PHENOXYETHANOL 0.5 0.5 0.5 1,2-HEXANEDIOL,CAPRYLYL GLYCOL 0.5 0.5 0.5 100 100 100

Preparation:

Add separately all ingredients of phase 1 to the water with stirring atroom temperature.

Mix well to obtain a homogenous phase.

Add the ingredients of phase 2 to phase 1, homogenize well understirring.

Add separately and slowly all the ingredients of phase 3 to phase1, 2.

Stir well till homogenously.

Continue in the same way adding phase 4 and 5 to phase 1, 2, 3.

Control homogeneity of the final product.

EXAMPLE 2

EYE CREAM A B C example 1 example 2 example 3 INCI Name/description ofingredients % % % Premix GLYCERIN 2.0 4.0 6.0 XANTHAN GUM 0.1 0.15 0.2Phase 1 WATER q.s. q.s. q.s. COLORANT ACID RED 27 0.0005 0.0007 0.0009FD & C YELLOW N°5 0.0005 0.001 0.0015 DISODIUM EDTA 0.035 0.040 0.0450CAFFEINE 0.3 0.2 0.25 PROPYLENE GLYCOL 1.5 1.5 2.3 CARBOMER 0.2 0.15 0.1Phase 2 GLYCERYL STEARATE 4.0 3.8 3.3 PEG-40 STEARATE 1.2 1.5 1.7SYNTHETIC BEESWAX 2.5 2.7 3.3 HYDROGENATED POLYISOBUTENE 1.0 2.0 2.7BUTYROSPERMUM PARKII (SHEA) 3.5 3.0 3.5 BUTTER STEARYL ALCOHOL &CETEARETH-20 4.3 4.6 4.8 Phase 3 CYCLOPENTASILOXANE & 1.5 3.5 2.5DIMETHICONOL CYCLOPENTASILOXANE & 2.5 1.5 2.5 CYCLOHEXASILOXANE Phase 4KAOLIN 0.7 1.0 1.5 NYLON-12 2.0 1.5 1.0 MICA & SILICA & TITANIUM DIOXIDE0.3 0.4 0.45 Phase 5 SODIUM HYDROXIDE (hydro) q.s. q.s. q.s. Phase 6KIGELIA AFRICANA FRUIT EXTRACT 0.1 2.0 0.6 (hydroglycolic) (Kigeline)GINGKO BILOBA LEAF EXTRACT 0.6 0.6 0.6 (hydroglycolic) AESCULUSHIPPOCASTANUM 0.4 0.4 0.4 (HORSE CHESTNUT) SEED EXTRACT (hydroglycerin)SECALE CEREALE (RYE) SEED 0.2 1.9 0.4 EXTRACT (hydro) (Coheliss ®Bio)MICROSPHERES SODIUM 0.05 0.12 0.08 HYALURONATE (Hyaluronic fillingspheres) ENCAPSULATED INGREDIENTS RPF 0.25 0.2 0.15 COMPLEX PEG-6ISOSTEARATE & HESPERETIN 0.2 0.2 0.2 LAURATE BIFIDA FERMENT LYSATE 0.23.0 0.6 3.0 0.2 2.0 GLYCERINS WATER & HYDROXYPROPYL CYCLODEXTRIN &PALMITOYL TRIPEPTIDE-38 (Matrixyl ®Synthe{grave over ( )}6 ™) 0.25 2.00.45 PIPER NIGRUM (PEPPER) SEED EXTRACT (hydro) (Retilactyl D ®) Phase 7FRAGRANCE 0.05 0.1 0.15 PHENOXYETHANOL, 0.5 0.5 0.5 1,2-HEXANEDIOL,CAPRYLYL GLYCOL 0.5 0.5 0.5 TOTAL 100 100 100

Preparation:

Dissolve the ingredients of phase 1 in the water and heat up toapproximately 75° C.

Heat separately phase 2 to approximately 75° C.

Prepare premix till homogeneity and add it to phase 1 stir well.

Put phase 2 in phase 1 under stirring and homogenizing.

Cool down the homogenous emulsion phase 1, 2 to 50-55° C.

Add phase 3 and 4 separately to phase 1, 2; continue stirring.

Neutralisation of phase 1, 2, 3, 4 with phase 5 under stirring.

Homogenize well.

Cool with gentle stirring below 40° C. and continue the adding of allingredients from phase 6 to phase 1, 2, 3, 4, 5, control homoneneity.

Cool again with gentle stirring till 20-25° C., add the ingredients ofphase 7 to phase 1, 2, 3, 4, 5, 6.

-   -   Control final homogeneity.

EXAMPLE 3

NIGHT CREAM A B C example 1 example 2 example 3 INCI Name/description ofingredients % % % Premix GLYCERIN 3.0 3.5 4.0 XANTHAN GUM 0.6 0.5 0.65Phase 1 WATER q.s. q.s. q.s. COLORANT ACID RED 27 0.0001 0.0002 0.0003BUTYLENE GLYCOL 2.0 1.5 2.5 Phase 2 CAPRYLIC/CAPRIC TRIGLYCERIDE 3.0 2.03.5 GLYCERYL STEARATE 2.5 2.2 1.85 CETYL ALCOHOL 4.0 3.5 4.3 ETHYLHEXYLPALMITATE 3.0 4.0 2.5 BUTYROSPERMUM PARKII (SHEA) 3.0 3.5 4.5 BUTTERSORBITAN TRISTEARATE 0.2 0.2 0.2 Phase 3 CYCLOPENTASILOXANE & 5.0 2.04.0 DIMETHICONOL Phase 4 METHYL METHACRYLATE 1.7 3.0 2.5 CROSSPOLYMERNYLON-12 1.0 1.5 2.0 Phase 5 KIGELIA AFRICANA FRUIT EXTRACT 0.2 3.0 0.6(hydroglycolic) (Kigeline) HYDROLYZED CITRUS AURANTIUM 2.0 1.2 0.6DULCIS FRUIT EXTRACT (hydro) MAURITIA FLEXUOSA FRUIT OIL 0.2 0.25 0.3SECALE CEREALE (RYE) SEED 0.25 2.3 0.6 EXTRACT (hydro) (Coheliss ®Bio)ENCAPSULATED INGREDIENTS RPF 0.25 0.2 0.15 COMPLEX DECARBOXY CARNOSINEHCL 0.25 0.1 0.15 (hydroglycolic) BIFIDA FERMENT LYSATE 0.3 3.5 0.55GLYCERINS WATER & 0.5 3.5 2.0 HYDROXYPROPYL CYCLODEXTRIN & PALMITOYLTRIPEPTIDE-38 (Matrixyl ®Synthe{grave over ( )}6 ™) PIPER NIGRUM(PEPPER) SEED 0.3 2.5 0.45 EXTRACT (hydro) (Retilactyl D ®) Phase 6FRAGRANCE 0.1 0.2 0.3 PHENOXYETHANOL 0.5 0.5 0.5 1,2-HEXANEDIOL,CAPRYLYL GLYCOL 0.5 0.5 0.5 HYDROXYETHYL ACRYLATE & SODIUMACRYLOYLDIMETHYL TAURATE COPOLYMER & SQUALANE 3.5 3.9 3.7 & POLYSORBATE60 TOTAL 100 100 100

Preparation:

Dissolve the ingredients of phase 1 in the water and heat up toapproximately 75° C.

Heat separately phase 2 to approximately 75° C.

Prepare premix till homogeneity and add it to phase 1 stir well.

Put phase 2 in phase 1 under stirring and homogenizing.

Cool down the homogenous emulsion phase 1, 2 to 50 - 55° C. withstirring.

Add phases 3 and 4 separately to phase 1, 2; continue stirring.

Homogenize well.

Cool with gentle stirring phase 1, 2, 3, 4 below 35° C. and continue theadding of all ingredients from phase 5 to phase 1, 2, 3, 4, controlhomoneneity.

Cool again with gentle stirring till 20-25° C., add the fragrance, thepreservatives and the blend of gel to the phase 1, 2, 3, 4, 5.

Control final homogeneity.

EXAMPLE 4 Test Protocol of HAS1 mRNA Expression Measurement

Normal human fibroblasts were treated by the active ingredient/mixtureof active ingredients diluted in the cell culture medium for 3 hours at37° C., 5% CO₂. Non treated cells were incubated in the same conditionswith cell culture medium.

The HAS1 mRNA expression was then assessed by using the Real-Time (RT)PCR (reverse transcription polymerase chain reaction) technology. Forthat purpose, total RNA were extracted with the ABI Prism 6100 Nucleicacid prepstation (Applied Biosystems) and quantified with aspectrophotometer at 260nm. First strand cDNA synthesis was performedusing 2 μg of total RNA with the High Capacity cDNA Archive kit.Real-Time PCR was carried out on 10 ng of cDNA with the 7300 Real TimePCR System by using the TaqMan primers and probe (Applied Biosystems)specific to HAS1. Amplification conditions were as follows: 15 sec at95° C., 1 min at 60° C. for 40 cycles. Relative changes in geneexpression were calculated according to the 2^(−ΔΔCT) method with the7300 System software™.

The results are demonstrated in following table 1 and in FIG. 1. FIG. 1shows the HAS1 mRNA expression measured with RT-PCR on normal humanfibroblasts.

TABLE 1 HAS1 mRNA expression Treatment 3 h Treatment 6 h StimulationStatis- Stimulation Statis- (%) tical (%) tical (/Untreated) analysis(/Untreated) analysis Synergy with 2 ingredients: Matrixyl 2% 127.2%s/untreated 59.6% s/untreated Retilactyl 0.5% 60.0% s/untreated 78.9%s/untreated Mix: Matrixyl 2% + 377.1% s/untreated 289.4% s/untreatedRetilactyl 0.5% s/each s/each ingredient ingredient s/untreated:statistically significant compared to untreated cells s/each ingredient:Mix statistically significant compared to each ingredient

As it can be taken from the results, the combination of 2%Matrixyl®Synthe{grave over ( )}6™ (which corresponds to 0,0005 wt %Palmitoyl Tripeptide-38) and 0,5% Retilactyl D® (which corresponds to0,0105 wt % Piper nigrum seed extract) increased the HAS1 mRNAexpression to 377,1%, whereas the HAS1 mRNA expression ofMatrixyl®Synthe{grave over ( )}6™ alone was 127,2% and that ofRetilactyl D® alone was 60%. Addition of 0,5% Coheliss®Bio (whichcorresponds to 0,03 wt % Secale cereale (rye) seed extract) and 0,5%Kigeline (which corresponds to 0,0054 wt % Kigelia africana fruitextract) further improved the HAS1 mRNA expression to 401,6%.

EXAMPLE 5 Exploration of the Activity of a Finished Product of theInvention on Hyaluronan on Living Human Skin Explant

The day cream C of Example 1 with 2% Matrixyl and 0,5% of each ofRetilactyl, Coheliss Bio and Kigeline was tested with regard to itsactivity on dermis hyaluronic acid synthesis.

The explants were kept in survival in BEM culture medium at 37° C. in ahumid, 5% CO₂ atmosphere. The product tested was applied on days D0, D2and D4 on the basis of 2 mg/cm². Half of the culture medium was renewedon D2 and D4. The control explant did not receive any treatment.

On D0, 3 explants were collected and cut in two parts. One half wasfixed in buffered formalin, and the other half was frozen at −80° C. OnD5, 3 explants treated or not with the product were collected andprocessed in the same way.

The frozen sample were cut into 7 μm thick sections using a Leica CM3050 cryostat. Immunostaining of HA was realized on frozen sections witha biotinylated HABP (Seikagaku ref 400763-1A) for 1 hour at roomtemperature. The staining was revealed using Streptavidin/FITC (Caltag,SA 1001).

The immunostaining was assessed by microscopical observation using Leicafilter block I3 (FITC) for HABP and by image analysis. It revealed thathyaluronic acid synthesis improved of 61%.

FIG. 2 shows the immunostaining results of this in vitro test on normalhuman skin. Hyaluronic acid is stained in green.

1-11. (canceled)
 12. A cosmetic composition for increasing hyaluronicacid synthesis in skin, the composition comprising: 0.000025 to 0.001weight percent of Palmitoyl-Lysyl-Dioxymethionyl-Lysine; 0.00105 to0.063 weight percent of Piper nigrum seed extract; water; andcosmetically acceptable auxiliaries, wherein all given percentagesrelate to a total weight of the composition.
 13. The compositionaccording to claim 12, comprising: 0.003 to 0.18 weight percent ofSecale cereale seed extract, wherein all given percentages relate to thetotal weight of the composition.
 14. The composition according to claim12, comprising: 0.000054 to 0.0324 weight percent of Kigelia africanafruit extract, wherein all given percentages relate to the total weightof the composition.
 15. The composition according to claim 12,comprising: 0.000125 to 0.00075 weight percent ofPalmitoyl-Lysyl-Dioxymethionyl-Lysine, wherein all given percentagesrelate to the total weight of the composition.
 16. The compositionaccording to claim 12, comprising: 0.0021 to 0.0315 weight percent ofPiper nigrum seed extract, wherein all given percentages relate to thetotal weight of the composition.
 17. The composition according to claim12, comprising: 0.006 to 0.09 weight percent of Secale cereale (rye)seed extract, wherein all given percentages relate to the total weightof the composition.
 18. The composition according to claim 12,comprising: 0.00108 to 0.0162 weight percent of Kigelia africana fruitextract, wherein all given percentages relate to the total weight of thecomposition.
 19. The composition according to claim 12, comprising: oneor more auxiliary substances selected from the group consisting ofemulsifiers, emollients, humectants, moisturizers, gelling agents,chelating agents, complexing agents, viscosity modulating agents,opacifiers, preservatives, colorants, fragrances, skin care agents,tanning agents, UV filters, buffers, solvents, and mixtures thereof. 20.The cosmetic composition according to claim 12, wherein the compositionis adapted for use in repairing or smoothing wrinkles.
 21. A method fortreating wrinkles on skin, the method comprising: applying the cosmeticcomposition according to claim 12 to skin areas with wrinkles to betreated.
 22. The cosmetic composition according to claim 21, wherein thewrinkles are facial wrinkles.
 23. The method according to claim 22,wherein the wrinkles are facial wrinkles.
 24. The composition accordingto claim 12, comprising: 0.006 to 0.09 weight percent of Secale cerealeseed extract, wherein all percentages relate to the total weight of thecomposition.
 25. The composition according to claim 12, comprising:0.00108 to 0.0162 weight percent of Kigelia africana fruit extract,wherein all given percentages relate to the total weight of thecomposition.
 26. The composition according to claim 13, comprising:0.000054 to 0.0324 weight percent of Kigelia africana fruit extract,wherein all given percentages relate to the total weight of thecomposition.
 27. The composition according to claim 12, comprising:0.00025 to 0.00075 weight percent ofPalmitoyl-Lysyl-Dioxymethionyl-Lysine, wherein all given percentagesrelate to the total weight of the composition.
 28. The compositionaccording to claim 12, comprising: 0.00042 to 0.021 weight percent ofPiper nigrum seed extract, wherein all given percentages relate to thetotal weight of the composition.
 29. The composition according to claim13, comprising: 0.006 to 0.09 weight percent of Secale cereale seedextract, wherein all given percentages relate to the total weight of thecomposition.
 30. The composition according to claim 13, comprising:0.00108 to 0.0162 weight percent of Kigelia africana fruit extract,wherein all given percentages relate to the total weight of thecomposition.